Phytochemical Analysis, Antidiabetic Potential and in-silico Evaluation of Some Medicinal Plants

Pharmacognosy Research,2021,13,3,140-148.DOI:10.5530/pres.13.3.6Published:June 2021Type:Original ArticleAuthors:Basanta Kumar Sapkota, Karan Khadayat, Bikash Adhikari, Darbin Poudel, Purushottam Niraula, Prakriti Budhathoki, Babita Aryal, Kusum Basnet, Mandira Ghimire, Rishab Marahatha, and Niranjan Parajuli Author(s) affiliations:Basanta Sapkota1,2, Khadayat1, Adhikari1, Poudel1, Niraula1, Budhathoki1, Aryal1, Basnet1, Ghimire1, Marahatha1, Parajuli1,* 1Biological Chemistry Lab, Central Department of Chemistry, Tribhuvan University, Kathmandu, NEPAL. 2Department Butwal Multiple Campus, Butwal, Abstract:Background: The increasing frequency diabetes patients the reported side effects commercially available anti-hyperglycemic drugs have gathered attention researchers towards search for new therapeutic approaches. Inhibition activities carbohydrate hydrolyzing enzymes is one approaches to reduce postprandial hyperglycemia by delaying digestion absorption carbohydrates. Objectives: objective study was investigate phytochemicals, antioxidants, digestive inhibitory effect, molecular docking potent extract. Materials Methods: In this study, we carry out substratebased α-glucosidase α-amylase activity Asparagus racemosus, Bergenia ciliata, Calotropis gigantea, Mimosa pudica, Phyllanthus emblica, Solanum nigrum along with determination total phenolic flavonoids contents. Likewise, antioxidant evaluated measuring scavenging DPPH radical. Additionally, antibacterial also studied Agar well diffusion method. Molecular bioactive compounds from B. ciliata performed via AutoDock vina. Results: M. P. emblica exhibit significant against IC50 (μg/ml) (2.24 ± 0.01, 46.19 1.06), (35.73 0.65, 99.93 0.9) (8.12 0.29, no activity) respectively indicating a good source isolating potential drug candidate diabetes. These plant extracts showed ranges 13.2 26.5 μg/mL Staphylococcus aureus Klebsiella pneumonia. Conclusion: extract appeared be inhibitor. Further research should carried characterize inhibitor compounds. Keywords:Diabetes, Medicinal plants, docking, α-Amylase, α-GlucosidaseView:PDF (520.27 KB)